Цитомегаловирус (CMV) и биопсия кишечника у пациентов ВЗК, до назначения курса лечения!

Может быть и у нас включат в диагностику — более детальное обследование биопата, хотя бы на герпес-вирусы? Если нет денег на это, то может быть шепнут на ухо, что такое обследование можно сделать?

ЗАКЛЮЧЕНИЯ: Количественная оценка ДНК CMV в биопсии кишечника — полезный диагностический инструмент и может предсказать реакцию на применение стероидов у больных с язвенным колитом (UC).

Diagnostic utility of quantitative cytomegalovirus DNA polymerase chain reaction in intestinal biopsies from patients with inflammatory bowel disease.

Paul M, et al. J Lab Physicians. 2018 Jan-Mar.

Authors

Author information

1
Department of Clinical Virology, Institute of Liver and Biliary Sciences, New Delhi, India.
2
Department of Clinical Research, Institute of Liver and Biliary Sciences, New Delhi, India.
3
Department of Pathology, Institute of Liver and Biliary Sciences, New Delhi, India.
4
Department of Hepatology, Institute of Liver and Biliary Sciences, New Delhi, India.

Citation

J Lab Physicians. 2018 Jan-Mar;10(1):38-43. doi: 10.4103/JLP.JLP_94_17.

Abstract

OBJECTIVES: Diagnostic utility of cytomegalovirus (CMV) DNA quantitative polymerase chain reaction (qPCR) in inflammatory bowel disease (IBD) has not been established. We aimed to compare diagnostic utility of qPCR for CMV in biopsy specimens with blood, serology, and histopathology.

MATERIALS AND METHODS: A total of 132 patients were included (92 ulcerative colitis [UC], 9 Crohn’s disease, and 31 unclassified IBD). Comparison between CMV IgM, CMV DNA qPCR in biopsy, in blood and histopathology was done. Positive result in any of the test was considered as CMV infection. Various risk factors for CMV association with IBD were analyzed.

RESULTS: 

Confirmed CMV infection was seen in 41 (31.1%) patients.

Diagnostic sensitivity of different assays was: DNA in biopsy seen in 37 (90.2%),

DNA in blood in 19 (46.3%), CMV IgM in 15 (36.5%), and histopathology in 8 (19.5%). Thirty-two UC cases were further followed up for a median time of 14.0 (R: 3-31) months. They were grouped as group I — biopsy and blood DNA both positive (14, 43.7%),

Group II — biopsy positive and blood negative (17, 53.1%), and

Group III — biopsy negative but blood positive (1, 3.1%).

CMV DNA viral load in Group I was significantly higher (mean: 4.2 ± 1.0 log10 copies/mg) than Group II (mean: 3.2 ± 0.6 copies/mg) and Group III (viral load: 2.69 log10 copies/ml), P < 0.001. Steroid refractoriness was seen more in Group I cases (n = 9) P < 0.001. A cutoff of ≥2.5 log10 copies/mg of DNA in tissue was predictive for steroid refractoriness (AUROC = 0.84).

CONCLUSIONS: 

Quantitation of CMV DNA in intestinal biopsy is a useful diagnostic tool and can predict response to steroid treatment in patients with UC.

PMID

29403203 []

PMCID

PMC5784291

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